Terminator

Part:BBa_K2386000:Design

Designed by: Craig Mendonca   Group: iGEM17_UConn   (2017-10-25)


VCP1 violaxanthin/chlorophyll terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 362
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 10


Design Notes

Biobrick prefix and suffix added to VCP1 gene before synthesis.


Source

IDT synthesis, from Nannochloropsis sp. Gene identified by Kilian et al.

Oliver Kilian, Christina S. E. Benemann, Krishna K. Niyogi, and Bertrand Vick High-efficiency homologous recombination in the oil-producing alga Nannochloropsis sp. PNAS 2011 108 (52) 21265-21269; published ahead of print November 28, 2011, doi:10.1073/pnas.1105861108

References